Re: [診斷] 第八集看完了

看板Doctor_k作者desirable (迷途小書僮)時間16年前 (2008/11/01 03:47)推噓2(2推 0噓 6→)

留言8則, 2人參與討論串8/9 (看更多)

※ 引述《jimmyray825 (JimmyRay)》之銘言： : 打開第一頁的反應 : 又是以棒球為主題的病患背景呀..XD : 第二篇又是壽司店...囧 : 第三篇名人的病患 : 第四篇又是拳王的橋段...囧 : 第五篇總算有一點主線劇情了@@ 唉……這一直都是K系列的風格呀…… 故事背景總有很多類似的案例，不過，也總有新的醫案就是，舊瓶裝新酒，也算是可以接受了。 : 醫學point..眼角膜治療那段.. : 我真的覺得那個村子的設備真的好先進呀..!! (汗) : ips細胞...我們現實的報紙好像沒有看到過...囧 : 那種應該是會寫在論文裡面才會提到的吧 QQ 我想，這是因為，IPS細胞是由日本人首先發表在世界首屈一指的Cell和Nature期刊上，在日本算是件大事吧？如果台灣哪天也有人發了一篇Cell或Nature，應該也會上報的~ 貼一下相關資訊： 2003年時，英國劍橋大學的 John Gurdon研究小組發現，如果將已完全分化的小鼠胸腺細胞注射至非洲爪蟾的卵母細胞時，卵母細胞的某種天然神奇配方會重新設定胸腺細胞並讓其開始表現胚胎幹細胞的特殊轉錄因子 - Oct4。這項突破性的研究開啟了胚胎幹細胞點石成金術的濫觴。 2006年時，日本京都大學山中伸弥（Shinya Yamanaka) 教授的研究小組進一步解開這個神奇配方，根據他們的研究結果，只需要將四種基因 - Oct3/4, Sox2, c-Myc, Klf4- 送入已完全分化的纖維母細胞，即可以把纖維母細胞重新設定變回幹細胞。他們將這種「返老還童」的重新設定細胞稱之為「誘導式多能性幹細胞」(induced pluripotent stem cells, iPS cells)。 iPS細胞的特質類似胚胎幹細胞，只要經由特殊訊號分子的引導，即可將它們轉換成為各種體細胞 (神經細胞或肝臟細胞)。更重要的是，移植到小鼠皮下組織的 iPS細胞也會導致畸胎瘤(teratomas)，這也是胚胎幹細胞的一個重要特質。 http://www.sciscape.org/news_detail.php?news_id=2242 Takahashi, K. et al. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell, 126(4), 663-76, (2006). 摘要： Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was dispensable. These cells, which we designated iPS (induced pluripotent stem) cells, exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Subcutaneous transplantation of iPS cells into nude mice resulted in tumors containing a variety of tissues from all three germ layers. Following injection into blastocysts, iPS cells contributed to mouse embryonic development. These data demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors. http://www.cell.com/retrieve/pii/S0092867406009767 Generation of germline-competent induced pluripotent stem cells. 摘要： We have previously shown that pluripotent stem cells can be induced from mouse fibroblasts by retroviral introduction of Oct3/4 (also called Pou5f1), Sox2, c-Myc and Klf4, and subsequent selection for Fbx15 (also called Fbxo15) expression. These induced pluripotent stem (iPS) cells (hereafter called Fbx15 iPS cells) are similar to embryonic stem (ES) cells in morphology, proliferation and teratoma formation; however, they are different with regards to gene expression and DNA methylation patterns, and fail to produce adult chimaeras. Here we show that selection for Nanog expression results in germline-competent iPS cells with increased ES-cell-like gene expression and DNA methylation patterns compared with Fbx15 iPS cells. The four transgenes (Oct3/4, Sox2, c-myc and Klf4) were strongly silenced in Nanog iPS cells. We obtained adult chimaeras from seven Nanog iPS cell clones, with one clone being transmitted through the germ line to the next generation. Approximately 20% of the offspring developed tumours attributable to reactivation of the c-myc transgene. Thus, iPS cells competent for germline chimaeras can be obtained from fibroblasts, but retroviral introduction of c-Myc should be avoided for clinical application. http://www.nature.com/nature/journal/v448/n7151/abs/nature05934.html : 最後 : 一也長超快的!! K2裡的時間應該是有在明顯的流動的幾乎是依照現實時間在流動的？ -- ※ 發信站: 批踢踢實業坊(ptt.cc) ◆ From: 220.131.229.115 ※ 編輯: desirable 來自: 220.131.229.115 (11/01 03:51)